肝原位模型通过将肝癌组织块直接在小鼠肝脏进行移植，建立肝癌原位模型，相对皮下成瘤模型，更加模拟肝癌的实际生长情况。

先制备皮下成瘤模型，取出皮下肿瘤，切成小块；通过外科手术，将肿瘤块接种到动物肝脏上，制备肝原位移植模型，通过借助小动物活体成像仪器观察动物肝脏上肿瘤的形成、增殖以及体内转移灶的发生、发展情况，评价细胞的肝原位成瘤能力以及在动物体内的转移能力，主要适用于肝癌在体内原位成瘤和转移方向的研究。可提供的建模细胞系有：肝癌等。

肝癌细胞感染携带有Luc的慢病毒，原位接种于4周龄裸小鼠肝脏，4周后，活体成像。可见裸鼠体内有肿瘤形成。

1.Zheng T et.al Gankyrin Promotes Tumor Growth and Metastasis Through Activation of IL-6/STAT3 Signaling in Human Cholangiocarcinoma.Hepatology.2014 Mar;59(3):935-46.

2.Xu J, et.al Microwave Responsive Nanoplatform via P-Selectin Mediated Drug Delivery for Treatment of Hepatocellular Carcinoma with Distant Metastasis.Nano Lett. 2019 May 8;19(5):2914-2927.

3.Malehmir M .et.al.Platelet GPIbα is a mediator and potential interventional target for NASH and subsequent liver cancer.Nat Med. 2019 Apr;25(4):641-655.

4.Ma M et.al.Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis.Hepatology. 2019 Mar 6.

5.Yue X et.al.Polymeric immunoglobulin receptor promotes tumor growth in hepatocellular carcinoma.Hepatology. 2017 Jun;65(6):1948-1962.

6.Ding W .et.al.Epithelial-to-mesenchymal transition of murine liver tumor cells promotes invasion.Hepatology. 2010 Sep;52(3):945-53.