GV358

Vector information:

General:11615bp

HIV-1_5_LTR, trunkHIV-1_3_LTR:835-1015

psi:1067-1204

RRE:1680-1913

ORF frame 1:1558-2445

Ubiquitin Promoter:2617-3833

3FALG:3880-3957

SV40 promoter:3964-4341

EGFP:4350-5069

IRES:5092-5676

Puromycin:5683-6282

WPRE:6309-6896

HIV-1_5_LTR, trunkHIV-1_3_LTR:7420-7600

pBR322_origin:10467-9848

Ampicillin:11482-10622

AmpR_promoter:11552-11524

CAG_enhancer:318-605

CMV_immearly_promoter:239-810


Primer locations and sequences:

Ubi-F(3756-3778):GGGTCAATATGTAATTTTCAGTG

FLAG-R-1(3963-3943):GCTAGCTCATTTGTCGTCATC

 

说明:由于载体图谱空间有限,部分元件未在图谱中体现