皮下成瘤

将肿瘤细胞接种于裸鼠皮下,通过观察裸鼠体内肿瘤的形成情况,从而评价出细胞的增殖能力;肿瘤方向研究的经典方法,常用于肿瘤细胞增殖能力的检测。

技术原理

将肿瘤细胞/组织接种于动物皮下,制备皮下成瘤模型,通过观察动物体内肿瘤的形成和增殖情况,评价细胞/组织的皮下成瘤及增殖能力,主要适用于肿瘤在动物体内增殖方向的研究。可提供的建模细胞系有:肝癌、肺癌、结肠癌、食管癌、胃癌、宫颈癌等。

结果展示


肿瘤细胞感染携带GFP慢病毒,接种于4W龄裸小鼠皮下,4W后活体成像,可见裸鼠皮下有瘤体形成。

肿瘤细胞感染携带GFP慢病毒,接种于4W龄裸小鼠皮下,4W后处死,剥去瘤体,可见裸鼠皮下有瘤体形成。

参考文献

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