SA-b-gal染色实验

SA-b-gal染色实验检测目的基因消减对细胞衰老的影响

技术原理

大多数正常细胞在经过有限次分裂后会进入衰老期,不能再在一些常规刺激的诱导下进行分裂。其细胞周期,基因和蛋白表达都发生了改变。衰老的细胞体积会变大,在pH6.0时会表达高酶活性的β-半乳糖苷酶。利用细胞衰老时产生的β-半乳糖苷酶催化底物后,在光学显微镜下对细胞进行拍照计数,从而反映处于衰老状态的细胞数量。

结果展示


通过SA-b-gal染色实验检测siRNA慢病毒感染后,实验组促进衰老样表型发生。

参考文献

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