RNA水平研究

细胞免疫荧光

利用荧光标记抗体与目的抗原的特异性结合,通过荧光显微镜对抗原处的荧光进行拍照 ,可知细胞中目的抗原的定位和表达情况。

技术原理

根据抗原抗体反应的原理,先将已知的抗原或抗体标记上荧光基团,利用这种荧光抗体(或抗原)作为探针检查细胞内的相应抗原(或抗体)。借助荧光显微镜观察荧光,从而确定细胞内抗原/抗体的性质,并对其进行定位。比较不同条件处理的实验组之间,细胞内蛋白的表达差异和细胞定位。

结果展示

目的细胞按实验设计组别进行药物处理后,收集细胞悬液,离心,用缓冲液洗涤,加细胞染色缓冲液重悬后,用PE标记的CD11b抗体进行染色,染色结束后,用细胞染色缓冲液洗涤、重悬,进行流式仪检测 (Millipore,Guava easyCyte HT),分析各实验组别中CD11b 阳性细胞百分比差异。

参考文献

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